Signal to background | µ (µm) | σ (µm) | Confidence | R2 |
|---|
No background | 1.07 (0.02) | 0.25 (0.01) | 0.81 (0.01) | 0.47 (0.09) |
1000:1 | 1.06 (0.02) | 0.26 (0.01) | 0.04 (< 0.01) | 0.54 (0.12) |
100:1 | 1.08 (0.01) | 2.42 (5.41) |  < 0.01 (< 0.01) | 0.36 (0.14) |
10:1†| 8.40 (5.46) | 9.03 (6.70) |  < 0.01 (< 0.01) | 0.33 (0.21) |
True value | 1.13 | Â | Â | Â |
- Deconvolved SD-40 PSFs collected with a 40x/1.3 objective were used to generate input images for CCC with 50 spatially correlated pairings at 1.13 µm correlation distance. Additional flat background was added to each input image prior to analysis. Results from the CCC plugin for each signal to background ratio are shown. All values are shown as Mean (SD) for n = 10 repeats. †indicates that this result had two repeats fail to fit a Gaussian curve to the data and is n = 8
