Colocalization by cross-correlation, a new method of colocalization suited for super-resolution microscopy

BMC Bioinformatics

Table 5 Effect of additional non-correlated particles on CCC results

Extra particles/µm³	µ (µm)	σ (µm)	Confidence	R²
0	1.07 (0.02)	0.25 (0.01)	0.81 (0.01)	0.57 (0.10)
6.97 × 10^–4	1.07 (0.02)	0.25 (0.03)	0.75 (0.01)	0.52 (0.12)
1.74 × 10^–3	1.06 (0.01)	0.24 (0.02)	0.65 (0.01)	0.54 (0.15)
3.49 × 10^–3	1.07 (0.01)	0.25 (0.03)	0.51 (0.01)	0.47 (0.16)
6.97 × 10^–3	1.07 (0.03)	0.24 (0.04)	0.30 (0.02)	0.43 (0.09)
1.39 × 10^–2	1.08 (0.05)	0.27 (0.07)	0.13 (0.01)	0.34 (0.12)
2.79 × 10^–2	0.95 (0.33)	1.85 (3.63)	0.04 (0.02)	0.28 (0.18)
True value	1.13

Deconvolved SD-40 PSFs collected with a 40x/1.3 objective were used to generate input images for CCC with 50 spatially correlated pairings at 1.13 µm correlation distance. Extra particles indicate the density of additional uncorrelated PSFs that were added to each input image prior to analysis. Results from the CCC plugin for each extra particle quantity are shown. All values are shown as Mean (SD) for n = 10 repeats

ISSN: 1471-2105