Colocalization by cross-correlation, a new method of colocalization suited for super-resolution microscopy

BMC Bioinformatics

Table 4 Effect of molecular density on CCC results

Particles/µm³	µ (µm)	σ (µm)	Confidence	R²
3.48 × 10^–4	1.07 (0.01)	0.25 (0.02)	0.81 (0.01)	0.52 (0.15)
1.39 × 10^–2	1.07 (0.01)	0.25 (0.01)	0.52 (0.01)	0.54 (0.08)
5.58 × 10^–2	1.07 (0.01)	0.26 (0.01)	0.21 (< 0.01)	0.55 (0.04)
0.22	1.07 (0.01)	0.26 (0.02)	0.06 (< 0.01)	0.56 (0.02)
0.87	1.07 (0.01)	0.28 (0.03)	0.02 (< 0.01)	0.58 (0.04)
1.74	1.08 (0.02)	5.71 (6.81)	0.01 (< 0.01)	0.45 (0.18)
True value	1.13

Deconvolved SD-40 PSFs collected with a 40x/1.3 objective were used to generate input images for CCC with various numbers of spatially correlated pairings, reported as molecular density per image, at 1.13 µm correlation distance. Results from the CCC plugin for each molecular density are shown. All values are shown as Mean (SD) for n = 10 repeats

ISSN: 1471-2105