Colocalization by cross-correlation, a new method of colocalization suited for super-resolution microscopy

BMC Bioinformatics

Table 3 Effect of particle distance on CCC results

True distance (µm)	µ (µm)	σ (µm)	Confidence	R²	PCC
0	0 (0)	0.20 (< 0.01)	0.99 (< 0.01)	0.84 (0.01)	0.79 (< 0.01)
0.3	0.02 (0.05)	0.27 (0.02)	0.98 (< 0.01)	0.85 (0.04)	0.22 (0.03)
0.45	0.34 (0.03)	0.21 (0.02)	0.96 (< 0.01)	0.81 (0.06)	0.06 (0.01)
0.6	0.49 (0.01)	0.23 (0.01)	0.94 (< 0.01)	0.72 (0.13)	0.02 (< 0.01)
1.13	1.06 (0.02)	0.24 (0.02)	0.81 (0.01)	0.54 (0.11)	0 (< 0.01)
2.26	2.24 (0.02)	0.32 (0.07)	0.55 (0.01)	0.33 (0.08)	0 (< 0.01)

Deconvolved SD-40 PSFs collected with a 40x/1.3 objective were used to generate input images for CCC with 50 spatially correlated pairings at various correlation distances. Results from the CCC plugin and Persons R value (PCC) for each distance are shown. All values are shown as Mean (SD) for n = 10 repeats

ISSN: 1471-2105