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Table 2 Effect of objective lens selection on CCC results

From: Colocalization by cross-correlation, a new method of colocalization suited for super-resolution microscopy

Magnification/NA

µ (µm)

σ (µm)

Confidence

R2

20x/0.75

0.03 (0.08)

4.48 (0.60)

0.14 (0.02)

0.57 (0.05)

25x/0.95

0.04 (0.08)

2.30 (1.07)

0.34 (0.09)

0.71 (0.10)

40x/1.1

0.90 (0.05)

0.74 (0.17)

0.65 (0.04)

0.72 (0.04)

40x/1.3

0.88 (0.02)

0.53 (0.05)

0.73 (0.02)

0.77 (0.09)

63x/1.3

0.90 (0.02)

0.50 (0.02)

0.82 (0.01)

0.80 (0.07)

True value

1.13

   
  1. Non-deconvolved PSFs collected using various objectives were used to generate input images for CCC with 50 spatially correlated pairings at 1.13 µm correlation distance. Results from the CCC plugin for each objective used are shown. All values are shown as Mean (SD) for n = 10 repeats
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BMC Bioinformatics

ISSN: 1471-2105

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