Colocalization by cross-correlation, a new method of colocalization suited for super-resolution microscopy

BMC Bioinformatics

Table 1 Effect of imaging modality on CCC results

Imaging modality	µ (µm)	σ (µm)	Confidence	R²
WF	0.83 (0.29)	1.27 (0.40)	0.42 (0.07)	0.70 (0.11)
SD-40	0.89 (0.02)	0.53 (0.04)	0.73 (0.01)	0.74 (0.08)
SD-25	0.89 (0.02)	0.58 (0.07)	0.63 (0.03)	0.68 (0.07)
Decon WF	1.01 (0.03)	0.32 (0.02)	0.79 (0.01)	0.65 (0.09)
Decon SD-40	1.05 (0.02)	0.26 (0.02)	0.87 (0.01)	0.47 (0.12)
Decon SD-25	1.07 (0.01)	0.24 (0.01)	0.81 (< 0.01)	0.57 (0.15)
iSIM	1.04 (0.02)	0.28 (0.02)	0.92 (< 0.01)	0.57 (0.13)
3D-STED	1.09 (0.01)	0.19 (0.01)	0.94 (< 0.01)	0.41 (0.15)
True value	1.13

PSFs collected from various microscope imaging modalities were used to generate input images for CCC with 50 spatially correlated pairings at 1.13 µm correlation distance. Results from the CCC plugin for each imaging modality are shown. All values are shown as Mean (SD) for n = 10 repeats

ISSN: 1471-2105