Fig. 2

Spatial correlation of mitochondria and ER. a Lateral cross-section of an MLE-12 cell stained for mitochondria (magenta) and ER (cyan). Scale bar indicates 5 µm for all images. b Cross-correlation image following removal of low spatial frequency contributions. (inset) Contrast enhanced and enlarged image of the center of the cross-correlation image. The dim pixels at the center indicate low correlation of untranslated images. c The blue line is the radial profile of the original cross-correlation, the green line is the radial profile after the pixel randomization procedure (see Implementation for details), and the red dashed line is the fit Gaussian curve. d Composite image of the data that contributed to the Gaussian result. ER near the cell periphery or otherwise distant from mitochondria are notably dimmer. The region of the nucleus was omitted from analysis using the input mask