Fig. 1

Visualization of protein folding energetics. \(\mathrm{\Delta \Delta }G\) is the difference between \({\Delta G}^{WT}\) and \({\Delta G}^{Mutant}\), and informs the stability effect of a missense mutation. A mutation is destabilizing if it results in higher energy when folded than the wild-type, and stabilizing if the mutant achieves lower energy when folded than the wild-type. The energy curve colored in red illustrates a case of a destabilizing mutation