Fig. 2

Sample results of msPIPE using the human WGBS dataset. a The read quality and statistics of all processed input samples were reported to the MultiQC html file. b The average CpG methylation levels in each genomic context, including promoter, gene, exon, intron, and intergenic regions of the old sperm sample are represented by a bar plot. The methylation levels (%) of c CpG, d CHG, e CHH context in the old sperm sample are shown. The bin size of the histogram is 10%. f The average levels of CpG, CHG, and CHH methylation for each given sample. g Genome-wide CpG methylation levels as well as UMR and LMR distribution in the old sperm sample are presented as the Circos plot. The red bar plot on the outermost track represents the average methylation level for 100 Kbp bin. In the absence of data, it was represented by a gray shadow. The dot plots on the inner two tracks represent UMR region shown in light green and the LMR region in light blue. The height of the graph indicates the methylation level of each region. A zero average methylation of the UMR (or LMR) is indicated by a red dot