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Fig. 6 | BMC Bioinformatics

Fig. 6

From: Annotation depth confounds direct comparison of gene expression across species

Fig. 6

Historical variation in human transcriptome annotation leads to variable abundance estimates for the same genes in the same sample. A Abundance and gene length variation in human transcriptome annotation are accompanied with better read mapping over time, consistent with the improvement of the representation of the human transcriptome. B Higher gene length in earliest examined version (v43) is consistent with lower tpm estimates, while this relationship is less consistent for genes with shorter length. C This reduced tpm is driven by the lack of increase in reads mapping to longer versions of current genes, which suggests that those lengths were artificially adding improper sequence, which was not part of expressed portion of the gene

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