Fig. 5

Estimates of tpm are relatively robust to reduction in reads mapped per gene, while read mapping increases lead to proportional tpm inflation. Few genes with high proportion of their length of transcripts represented present with higher reads mapped compared to full representation transcriptome. This could be due to lower competition for reads from similar transcripts in paralogous genes that are poorly annotated in pre-clinical species. This inflation of mapped reads leads to inflation of tpm estimates, suggesting a potential issue in direct abundance comparisons between species with vastly divergent genome annotations