Concatenation of paired-end reads improves taxonomic classification of amplicons for profiling microbial communities

Table 1 Bacterial mock communities used in pipeline analyses

Study ID^a	Amplicon	Mock type	No. isolates^b	No. Genera	No. read pairs	Read length	R1 trimmed-length	R2 trimmed-length	Avg. Q R1	Avg. Q R2
A1	V1-3	Balanced	19	19	950,404	301	301	225	34	29
C1	V4	Unbalanced	27	11	8,161,940	251	235	217	33	29
G1	V4	Balanced	20	17	1,426,252	301	301	258	34	32
G2	V4	Balanced	20	17	828,788	301	299	207	33	27
G3	V4	Unbalanced	20	17	1,346,668	301	301	229	34	31
G4	V4	Unbalanced	20	17	832,756	301	299	209	33	27
S1	V4	Balanced	49	37	3,722,660	250	240	220	36	36
S2	V4	Unbalanced	49	37	2,103,960	250	240	220	36	35
S3	V3-4	Balanced	49	37	357,444	250	240	220	35	35
S4	V3-4	Balanced	49	37	1,644,752	250	240	220	36	35

^aSequence information can be found in a separate table (Additional file 3: Table S1)
^bMock community composition can be found in a separate table (Additional file 3: Table S2)

ISSN: 1471-2105