Fig. 2

The successive steps of an EvoLaps analysis: a first step of the analysis: submission on the fly of a consensus of a phylogeographic reconstruction (NEXUS format), including a newick string for the phylogenetic tree and the latitude/longitude coordinates for each node of the tree (sample and ancestral nodes). b server side: the submitted file is parsed to extract and produce JSON files for the tree and the coordinates which are returned to display the tree and the samples on the geographic map; c second step of the analysis: clustering of the sample and/or ancestral locations on the geographic map with the help of several methods: K-means algorithm and/or lasso selection and/or clade selection or meridian/parallel grid, clusters are submitted to the server and d given a chosen color scale, the tree is color-encoded, the transition diagram is computed in a top-down reading of the color-encoded tree, and is displayed; e step three of the analysis: edition of the paths on the geographic map in a top-down reading of the transition diagram. The analysis can be iterated gradually from step two to refine the phylogeographic pattern