Fig. 4

Comparison of normalization efficiency by CHIPIN and the three other methods in genomic regions corresponding to differentially expressed genes in the shSpi1-A2B cells (across replicates). A H3K27ac density profiles around TSSs of genes down- and up-regulated by Spi1 in two conditions: “Spi1++”—Spi1 overexpressed, “Spi1−”—Spi1 repressed. B Differences in H3K27ac signal in zones 1–3 (Fig. 1E) in gene promoters for genes up- and down-regulated by Spi1; axes show differences between density values in conditions Spi1++ and Spi1−. Correct normalization procedures would result in observations laying close to the diagonal (\(y = x\), grey dotted line)