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Table 5 Quantification results for the Klyo samples using the reference dataset AFS20 and the average result for AFS31RS90

From: A big data approach to metagenomics for all-food-sequencing

Dataset

Classifier

Cattle

Pig

W.Buf.

Goat

Chicken

Turkey

Σ FP

Σ Dev

KLyo_A

Expected

14.0%

80.0%

0.00%

0.00%

0.50%

5.50%

  
 

AFS-MC

16.6%

71.5%

0.04%

0.02%

0.60%

4.64%

0.28%

12.39%

 

MCSpark

16.9%

71.2%

0.04%

0.02%

0.60%

4.64%

0.32%

12.99%

 

CLARK

16.4%

70.4%

0.20%

0.09%

0.62%

4.61%

0.51%

13.55%

 

Kraken2

15.9%

70.0%

0.27%

0.11%

0.65%

4.59%

0.87%

13.82%

 

K2+Brack

17.6%

70.3%

0.30%

0.14%

0.66%

4.63%

0.97%

15.33%

KLyo_B

Expected

36.0%

58.0%

0.00%

0.00%

2.00%

4.00%

  
 

AFS-MC

37.6%

51.0%

0.12%

0.04%

2.05%

2.99%

0.50%

10.16%

 

MCSpark

37.9%

50.5%

0.12%

0.04%

2.06%

3.02%

0.60%

11.11%

 

CLARK

35.9%

50.4%

0.47%

0.19%

2.10%

3.01%

1.03%

9.84%

 

Kraken2

34.5%

49.9%

0.68%

0.24%

2.12%

2.99%

1.57%

12.11%

 

K2+Brack

39.1%

50.2%

0.32%

0.78%

2.15%

3.02%

1.84%

13.93%

KLyo_C

Expected

58.0%

36.0%

0.00%

0.00%

4.00%

2.00%

  
 

AFS-MC

57.7%

27.1%

0.16%

0.06%

3.56%

1.16%

0.95%

11.47%

 

MCSpark

57.7%

26.9%

0.16%

0.06%

3.63%

1.18%

0.95%

11.48%

 

CLARK

54.1%

25.9%

0.69%

0.29%

3.58%

1.16%

1.88%

17.11%

 

Kraken2

52.2%

25.7%

0.95%

0.36%

3.57%

1.17%

2.58%

19.94%

 

K2+Brack

58.6%

25.8%

1.07%

0.46%

3.60%

1.18%

2.89%

14.90%

KLyo_D

Expected

80.0%

14.0%

0.00%

0.00%

5.50%

0.50%

  
 

AFS-MC

74.7%

10.9%

0.23%

0.08%

4.66%

0.33%

0.93%

10.27%

 

MCSpark

74.7%

10.8%

0.23%

0.08%

4.69%

0.33%

1.09%

10.58%

 

CLARK

70.8%

10.8%

0.94%

0.39%

4.73%

0.35%

1.94%

15.27%

 

Kraken2

68.0%

10.7%

1.26%

0.48%

4.70%

0.36%

2.42%

18.62%

 

K2+Brack

77.6%

10.8%

1.45%

0.62%

4.76%

0.36%

2.87%

9.35%

Average

AFS-MC

  

0.14%

0.05%

  

0.67%

11.07%

 

MCSpark

  

0.14%

0.05%

  

0.74%

11.54%

 

CLARK

  

0.58%

0.24%

  

1.34%

13.94%

 

Kraken2

  

0.79%

0.30%

  

1.86%

16.12%

 

K2+Brack

  

0.71%

0.50%

  

2.14%

13.38%

AFS31RS90

AFS-MC

      

0.58%

13.97%

Average

MCSpark

      

0.59%

14.08%

  1. AFS-MC: AFS-MetaCache, MC-Spark: MetaCacheSpark, K2+Brack: Kraken2 with subsequent Bracken, W.Buf: Water Buffalo, Σ FP: Sum of all false positive read classifications, Σ Dev: Sum of absolute deviations to the given meat composition (best results for each dataset in bold)