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Fig. 3 | BMC Bioinformatics

Fig. 3

From: Family reunion via error correction: an efficient analysis of duplex sequencing data

Fig. 3

Distribution of SSCS family sizes with and without error correction. Single stranded consensus sequences (SSCS) are created when reads with identical barcodes are bundled together. A common practice requires at least three reads with identical barcodes to form a SSCS. Without error correction (0 mismatches) there is a striking abundance of singletons: single reads with a barcode that is different from all other barcodes in the sample. Applying error correction with progressively higher number of allowed mismatches (from 1 to 3) significantly decreases the number of singletons by re-uniting them with other reads

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