Fig. 3From: Family reunion via error correction: an efficient analysis of duplex sequencing dataDistribution of SSCS family sizes with and without error correction. Single stranded consensus sequences (SSCS) are created when reads with identical barcodes are bundled together. A common practice requires at least three reads with identical barcodes to form a SSCS. Without error correction (0 mismatches) there is a striking abundance of singletons: single reads with a barcode that is different from all other barcodes in the sample. Applying error correction with progressively higher number of allowed mismatches (from 1 to 3) significantly decreases the number of singletons by re-uniting them with other readsBack to article page