Table 2 Performance of ELMSI for longer micro-satellites classification

Tumor purity

0.9

0.7

No.

Breakpoint

Unit

μ_N

μ_T

Breakpoint

μT′

MSI

Breakpoint

μT′

MSI

1

34489

TCATT

86

125

34491

146.84

1

34489

163.44

1

2

122387

GGCC

425

525

122389

685.63

1

122387

724.23

1

3

189108

GCTAC

46

120

189158

105.65

1

189108

133.03

1

4

190653

CATC

43

136

190655

130.52

1

190653

170.21

1

5

194236

AAC

89

166

194238

145.93

1

194236

151.43

1

6

251655

GCT

71

111

251654

91.17

1

251655

71.71

1

7

311313

ACCA

56

236

311315

321.08

1

311313

331.60

1

8

356789

GCT

76

256

356790

51.47

1

356789

161.35

1

9

398971

TTCG

45

225

398973

213.12

1

398971

251.42

1

10

412340

G

100

280

412505

88.05

1

412340

70.50

1

11

432344

TGA

78

258

432343

177.30

1

432344

220.54

1

12

473174

AAGG

221

354

473176

462.75

1

473174

403.21

1

13

501994

CGCCG

78

161

501996

128.26

1

501994

329.52

1

14

505733

ACAGGG

40

111

505791

222.26

1

505733

248.28

1

15

526358

GTCC

58

144

526360

167.58

1

526358

152.30

1

16

612344

TGC

90

270

612342

355.32

1

612344

343.93

1

17

622735

GGTTC

77

142

622737

114.59

1

622735

197.75

1

18

677621

TCA

70

200

677623

163.89

1

677621

202.36

1

19

712345

GACT

89

269

712337

N/A

0

712345

230.62

1

20

731506

GA

146

203

731506

104.14

1

731506

88.48

1

21

776166

TAA

213

324

776167

359.1743

1

776166

564.01

1

22

842735

CTC

134

211

842734

213.29

1

842735

236.04

1

23

866450

TG

185

220

866450

371.53

1

866450

526.3551

1

24

891334

TCAGC

105

285

891336

234.20

1

891334

338.38

1

25

908385

AGAAT

167

229

908386

194.85

1

908385

294.27

1

26

910124

C

205

301

910204

98.17

1

910124

32.50

1

27

929056

CCG

120

210

929058

199.72

1

929056

202.66

1

28

944729

GGACT

90

190

944731

214.33

1

944729

225.51

1

29

964608

AGGGGG

56

156

964610

305.59

1

964608

296.61

1

30

973099

GGGCAC

355

460

973101

849.18

1

973099

N/A

0

Accuracy

0.967

0.967

Tumor purity

0.5

0.3

0.1

No.

Unit

Breakpoint

μT′

MSI

Breakpoint

μT′

MSI

Breakpoint

μT′

MS

1

TCATT

34491

155.09

1

34489

165.59

1

34489

272.51

1

2

GGCC

122389

354.08

1

122387

710.49

1

122387

619.04

1

3

GCTAC

189108

177.33

1

189108

127.27

1

189108

94.68

1

4

CATC

190655

137.45

1

190653

84.00

1

190653

19.35

1

5

AAC

194238

172.94

1

194236

125.12

1

194236

82.57

1

6

GCT

251654

86.32

1

251655

30.28

1

251655

N/A

0

7

ACCA

311355

240.46

1

311313

361.34

1

311313

509.08

1

8

GCT

356790

303.44

1

356789

189.28

1

356789

584.24

1

9

TTCG

398973

275.14

1

398971

282.36

1

398971

295.25

1

10

G

412483

66.43

1

412340

105.09

1

412340

N/A

0

11

TGA

432343

292.88

1

432344

270.84

1

432344

345.93

1

12

AAGG

473176

434.98

1

473174

640.55

1

473174

779.62

1

13

CGCCG

501996

278.35

1

501994

365.00

1

501994

800.13

1

14

ACAGGG

505844

246.03

1

505733

178.62

1

505733

339.87

1

15

GTCC

526361

120.87

1

526358

132.08

1

526358

112.71

1

16

TGC

612419

467.03

1

612344

570.02

1

612344

N/A

0

17

GGTTC

622737

215.57

1

622735

241.85

1

622735

254.75

1

18

TCA

677623

226.32

1

677621

241.83

1

677621

220.82

1

19

GACT

712347

393.39

1

712345

327.39

1

712345

N/A

0

20

GA

731506

77.51

1

731506

51.49

1

731506

N/A

0

21

TAA

776167

405.81

1

776166

170.12

1

776166

340.20

1

22

CTC

842734

221.52

1

842735

321.37

1

842735

204.91

1

23

TG

866450

485.10

1

866450

870.52

1

866450

236.77

1

24

TCAGC

891336

316.81

1

891334

112.10

1

891334

677.12

1

25

AGAAT

908386

206.39

1

908385

457.04

1

908385

152.63

1

26

C

910220

N/A

0

910124

N/A

0

910124

N/A

0

27

CCG

929058

203.18

1

929056

98.56

1

929056

269.48

1

28

GGACT

944731

257.57

1

944729

250.61

1

944729

279.33

1

29

AGGGGG

964610

416.61

1

964608

380.03

1

964608

691.83

1

30

GGGCAC

973101

1182.00

1

973099

N/A

0

973099

748.71

1

Accuracy

0.967

0.933

0.8