Fig. 1

Schematic of the DryMass pipeline (shaded area) for extracting radius and refractive index (RI) of spherical objects. a Four HL60 cells are shown in a split-view. The left part of the image represents the raw hologram data. The right part of the image has been converted to quantitative phase data via an automated Fourier analysis. Regions of interest (ROIs) are found (white rectangles) and background-corrected for subsequent analysis steps. The cell in ROI 4 is also shown in B and D. b Under the assumption of sphericity, the radius r and the RI n are extracted by fitting the Rytov model (black) to the phase data (orange). (c) Alternatively, ROI data can be analyzed by other software. d Visualization of the analysis results with the Rytov approximation. The orange dashed line indicates the position of the line profile shown in (B). The white dashed circle highlights the fitted perimeter of the cell. Other parameters, such as the acquisition time t, are also retained. e For each ROI, all relevant parameters are compiled (blue data point indicates the exemplary ROI). f Final analysis steps can be performed with other software. The Fiji logo was kindly provided by the ImageJ community. The R-project logo is licensed CC BY-SA 4.0