Skip to main content
Fig. 2 | BMC Bioinformatics

Fig. 2

From: Guide for library design and bias correction for large-scale transcriptome studies using highly multiplexed RNAseq methods

Fig. 2

Library bias correction to the BEAS-2B libraries. a Correlation between the sequencing depths (x-axis) and the raw read counts (y-axis) of CCDC85B (left) and RRM2 (right) before (top) and after NBGLM-LBC (bottom); solid lines were the linear regressions, and the transparent bands were the 95% prediction intervals. b Spearman correlation coefficients between the replicates after NBGLM-LBC. c PCA on VST normalized expression levels after the library bias correction. d Relation between the library quantity (x-axis of the left panel), proportions of mapped reads on protein coding genes (x-axis of the right panel), and the library redundancy (y-axis)

Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us