Prediction of regulatory targets of alternative isoforms of the epidermal growth factor receptor in a glioblastoma cell line

Table 3 Primer sequences for qPCR

Traget mRNA	Label	Sequence 5^′→3^′	Localization	Corresponding mRNA
ALDH4A1	Sense	AGTGGGACTTTGGCTGATCC	128-147	NM_170726.2
	Antisense	GTGAAGGCTAAGACGGGCTC	398-379
CKAP2L	Dense	ACATCAGTGGAAGAGCTGGC	1940-1959	NM_152515.4
	Antisense	TTCTGCCTTGGCTATTCGGG	2044-2025
CLCA2	Sense	CCATTGCCCTGGGTTCATCT	1690-1709	NM_006536.6
	Antisense	GGCCTGCCACGTAACTAGAA	1961-1942
EGFR all	Sense	TCAGCCTCCAGAGGATGTTC	392-411	NM_005228.3
	Antisense	GTGTTGAGGGCAATGAGGAC	511-530
EGFR v1	Sense	CCCAGTACCTGCTCAACTGG	2689-2709	NM_005228.4
	Antisense	TAGGCACTTTGCCTCCTTCTG	2889-2869
EGFR v4	Sense	GCCATCCAAACTGCACCTAC	2105-2126	NM_201284.1
	Antisense	GGACACGCTGCCATCATTAC	2211-2192
GALNS	Sense	CAGCTGTTGCTGGTGCTCAG	123-142	NM_000512.4
	Antisense	AGTTTGGGAAAAGCAGCCCT	303-284
GAPDH	Sense	CACCCACTCCTCCACCTTTG	943-962	NM_002046.7
	Antisense	CCACCACCCTGTTGCTGTAG	1052-1033
HPRT	Sense	TTGCTGACCTGCTGGATTAC	391-410	NM_000194.2
	Antisense	CTTGCGACCTTGACCATCTT	652-633
MMP2	Sense	CCCTCGCAAGCCCAAGTGGG	650-669	NM_004530.5
	Antisense	CCATGCTCCCAGCGGCCAAA	848-828
ROCK1	Sense	GGTGCTGGTAAGAGGGCATT	905-924	NM_005406.2
	Antisense	CGCAGCAGGTTGTCCATTTT	997-978
TPR	Sense	GCTGAGGGTGGACTCGATTT	115-134	NM_003292.2
	Antisense	AGACTTGGGCAGCTTGTTCA	357-338

ISSN: 1471-2105