Fig. 5From: In silico analysis of missense mutations in exons 1–5 of the F9 gene that cause hemophilia BComparison of the local environment of severe and mild mutations in the EGF domains of FIX. The protein backbone is shown in silver ribbons, interacting amino acids as a black licorice and the calcium ion as a white sphere. a, c, e, g, i correspond to wild type FIX. b, d, f, h, j correspond to mutant FIX. a Location of Gly105 in EGF-1 (from PDB ID 1PFX); the N-terminus of the domain is labeled. b Location of Asp105 in EGF-1; the N-terminus of the domain is labeled c Neighboring residues for Gln143 (from PDB ID 6MV4), labeled. d Neighboring residues for Arg143, clashing with the disulfide bond between Cys157 and Cys170, Tyr161 and Phe423. e Salt bridge between Glu124 in EGF-1 and Arg140 in EGF-2; neighboring positive residue also labeled (from PDB ID 1PFX). f Group of nearby positive charges in the Glu124Lys mutant. g Selected residues close to Val153 (from PDB ID 1PFX). h Residues that clash with Met153. i Residues coordinating the calcium ion in EGF-1; the residues that contribute their side chains are labeled (from PDB ID 1EDM). j Location of Pro96 as a first coordination shell residue for calciumBack to article page