Table 2 Top ideal GRIBCG-generated sgRNAs for each chromosome in A. thaliana and C. elegans
From: GRIBCG: a software for selection of sgRNAs in the design of balancer chromosomes
Organism | Chromosome | sgRNA Sequences | SSV | Average On-Target | CFD | Number of Cuts | Coverage (%) |
|---|---|---|---|---|---|---|---|
A. thaliana | 1 | TATGGCTTAAAATTTTATGTGGG | 0.341 | 0.821 | 2.4 | 7 | 82 |
A. thaliana | 2 | TTTAACCCACGGTATACCGCGGG | 0.577 | 0.754 | 1.3 | 4 | 75 |
A. thaliana | 3 | CAGATAGAGAGAAAGAGAGAGGG | 0.252 | 0.909 | 3.3 | 6 | 83 |
A. thaliana | 4 | AGGGCAAAAAAAAAAAACAGAGG | 0.325 | 0.965 | 2.7 | 5 | 88 |
A. thaliana | 5 | GGAGTAGGAGGAAGAGGAGGAGG | 0.385 | 0.962 | 2.6 | 7 | 100 |
C. elegans | I | GAGGGGGGGCCATACTAATAGGG | 0.958 | 0.642 | 1.0 | 4 | 96 |
C. elegans | II | TAGTGAAATTTTCATGAAAATGG | 0.786 | 0.587 | 1.0 | 4 | 79 |
C. elegans | III | CCTGGAGCTCCTGGAGCTCCTGG | 0.949 | 0.675 | 1.0 | 4 | 95 |
C. elegans | V | TGGAATAAAAATCTCAGAAATGG | 0.757 | 0.807 | 1.0 | 4 | 76 |
C. elegans | X | TACATTTTAATTTTAAAAAAAGG | 0.903 | 0.522 | 1.0 | 4 | 90 |