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Table 3 Estimated frequencies of three kangaroo sub-samples among the mixture of reads [8] for three amplicons resulted from our method

From: HaploJuice : accurate haplotype assembly from a pool of sequences with known relative concentrations

Amplicon

Target proportions

Average estimated proportions (average variation in %)

 

f 1

f 2

f 3

f 1

f 2

f 3

Amplicon 1

0.625

0.250

0.125

0.656

0.229

0.115

    

(4.9%)

(8.3%)

(8.0%)

Amplicon 2

0.625

0.250

0.125

0.640

0.246

0.114

    

(2.4%)

(1.6%)

(8.7%)

Amplicon 3

0.625

0.250

0.125

0.646

0.251

0.103

    

(3.4%)

(0.3%)

(17.9%)

  1. It revealed the existence of variations on the ratios of the sub-samples when mixing them during the library preparation. Ten data sets were for each amplicon