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Table 6 The efficiency of tandem repeats reconstruction from simulated data

From: De novo assembly of bacterial genomes with repetitive DNA regions by dnaasm application

Sequence len. [bp]

Motif len. [bp]

Min. cov. (Eq. 5)

Unitigs

Scaffolds

   

ABySS

Velvet

SPAdes

dnaasm

ABySS

Velvet

SPAdes

dnaasm

200

100

26

0/0/0

0/0/0

2/2/2

2/2/2

2/0/2

2/2/2

2/2/2

2/2/2

300

100

44

0/0/0

0/0/0

0/0/0

4/3/3

0/0/2

0/0/0

2/2/2

4/3/3

400

100

62

0/0/0

0/0/0

0/0/0

4/4/4

0/0/2

0/0/0

2/2/2

4/4/4

500

100

80

0/0/0

0/0/0

0/0/0

6/6/5

0/0/2

0/0/0

2/2/2

6/6/5

400

200

31

0/0/0

0/0/0

2/2/2

2/2/2

0/0/0

2/2/2

2/2/2

2/2/2

600

200

49

0/0/0

0/0/0

0/0/0

4/3/3

0/0/0

0/0/0

2/2/2

4/3/3

800

200

66

0/0/0

0/0/0

0/0/0

3/4/4

0/0/0

0/0/0

2/2/2

3/4/4

1000

200

84

0/0/0

0/0/0

0/0/0

6/6/5

0/0/0

0/0/0

2/2/2

6/6/5

600

300

32

0/0/0

0/0/0

2/2/2

2/2/2

0/0/0

0/0/0

2/2/2

2/2/2

900

300

50

0/0/0

0/0/0

0/0/0

3/3/3

0/0/0

0/0/0

2/2/2

3/3/3

1200

300

68

0/0/0

0/0/0

0/0/0

4/4/4

0/0/0

0/0/0

2/2/2

4/4/4

1500

300

86

0/0/0

0/0/0

0/0/0

5/5/5

0/0/0

0/0/0

2/2/2

5/5/5

800

400

33

0/0/0

0/0/0

2/2/2

2/2/2

0/0/0

0/0/0

2/2/2

2/2/2

1200

400

51

0/0/0

0/0/0

0/0/0

3/3/3

0/0/0

0/0/0

0/0/0

3/3/3

1600

400

69

0/0/0

0/0/0

0/0/0

4/4/4

0/0/0

0/0/0

0/0/0

4/4/4

2000

400

87

0/0/0

0/0/0

0/0/0

5/5/5

0/0/0

0/0/0

0/0/0

5/5/5

1000

500

33

0/0/0

0/0/0

2/2/2

2/2/2

0/0/0

0/0/0

2/2/2

2/2/2

1500

500

51

0/0/0

0/0/0

0/0/0

3/3/3

0/0/0

0/0/0

0/0/0

3/3/3

2000

500

69

0/0/0

0/0/0

0/0/0

4/4/4

0/0/0

0/0/0

0/0/0

4/4/4

2500

500

87

0/0/0

0/0/0

0/0/0

5/5/5

0/0/0

0/0/0

0/0/0

5/5/5

  1. The numbers in the table depict number of motif repetitions in reconstructed DNA sequence for depth of coverage 50x, 100x and 150x, respectively. The proper restorations are in bold, the expected number of motif repetitions is defined as in Table 4. Only our algorithm was able to reconstruct tandem repeats with more than two motif repetition. It is worth paying attention to dnaasm results for 100 bp and 200 bp motifs, where increasing the depth of coverage increases the probability of tandem repeat correct reconstruction