Fig. 2

ROI Pore Occupancy. ROI read distribution across 512 sequencing channels. Assuming that sequencing is a stochastic process, we should expect a Poisson distribution of reads/channel. However, we discovered that overly productive channels not fitting the expected distribution model (e.g., up to 191 reads/channel, black boxes) produced spurious reads not belonging to the carrier, target, or known contamination. Here, channels producing more than 7 reads were identified as HQNR-associated