Fig. 2

FPKM-scaled retention levels of U12-type and U2-type introns across various samples in MDS data, excluding transcripts that feature only introns with low average read counts over all samples (i.e. 1 read or less). a Boxplot showing FPKM-scaled retention levels of the U12-type introns (middle) as compared to their upstream and downstream U2-type intron. The thick horizontal lines in boxplots represents the median values and the whiskers represent 1.5 times the interquartile range. The box extends from the first quartile to the third quartile. b Boxplot showing the distribution of the FPKM-scaled retention levels of U12-type introns compared to the U2-type intron in ZRSR2mut, ZRSR2wt, and HEALTHY samples. c Density plot illustrating the frequency of the fold change (log2) of the retention levels of U12-type introns, randomly picked U2-type introns, U2-type introns upstream and downstream of the U12-type introns when comparing ZRSR2mut to the control samples of the MDS data. d Density plot illustrating the frequency of the ΔΨ values (increase of percentage spliced in) of the U12- and U2-type introns when comparing ZRSR2mut to the control samples. The Ψ values are between -1 and 1