Fig. 1

General project workflow. The general pipeline of comparing, aligning and synchronizing cell lines between EFO (version 2.85) and CLO (version 2.1.106) with additional information downloaded from Cellosaurus (version 22.0).The workflow was separated into four major steps (indicated as blue circles). Step 1: Cell lines and related biological information were downloaded and extracted from EFO, CLO and Cellosaurus. Step 2: Comparison and alignment of EFO cell lines to CLO through three intermediate processes: (i) EFO-Cellosaurus-CLO Mapping that performed cross-references and validations among the three cell line resources; (ii) Direct EFO-CLO Mapping that compared and mapped EFO cell lines to all CLO cell lines; (iii) Identification of EFO unique Cell Lines that were immortalized permanent cell lines not available in CLO. The results of (i)-(iii) were summarized in Fig. 4.EFO cell lines with foreign (non-EFO) namespace such as Brenda Tissue Ontology (BTO), stem cell lines and primary cell lines were excluded from the mapping and remained in EFO. The overall mapping result was summarized in Table 1. Step 3: The mapped EFO-CLO cell lines and EFO unique immortalized permanent cell lines would be merged or added to CLO. Step 4: The updated and synchronized CLO will later be imported to EFO immortalized permanent cell line module