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Table 10 Summary of radius results extracted from CLSM by eight algorithms and compared to the reference radius measurements from SEM

From: Modeling, validation and verification of three-dimensional cell-scaffold contacts from terabyte-sized images

Fluorescent Input

Radius ± stdev Estimate [um]

Number of points

Algorithm

ALL: Average ± stdev

Internal: Average ± s tdev

ALL

Internal

A1 (stats: single pixel)

2.2795 ± 0.6151

2.3075 ± 0.7189

32,608

22,128

A2 (stats: mixed pixel)

1.1190 ± 0.3128

1.1562 ± 0.3464

32,608

22,128

A4 (stats: noise model)

2.6851 ± 1.3196

2.7745 ± 1.5440

32,608

22,128

A5 (stats: MRF)

1.7532 ± 0.3353

1.7648 ± 0.3843

32,608

22,128

A6 (geom: σ= 1.0)

1.1139 ± 0.1960

1.0815 ± 0.2112

36,249

25,709

A7 (geom: σ= 1.5)

1.6251 ± 0.1065

1.6136 ± 0.1175

30,105

20,064

A8 (geom: thresh)

2.0404 ± 0.3567

2.0674 ± 0.4018

32,138

21,691

SEM Input

Average Radius: 1.1242 ± 0.075

Number of points: 104,341