Table 1 Test groups for the evaluation of the proposed methods
From: A multiplex primer design algorithm for target amplification of continuous genomic regions
Short240: 240 s time limit with short initial candidate sequence (120 bases) | Short480: 480 s time limit with short initial candidate sequence (120 bases) | Long240: 240 s time limit with long initial candidate sequence (240 bases) | |
|---|---|---|---|
bothNormal: Pforward and Preverse in normal order by base location | 1. test group for 2 to 5 tubes | 2. test group for 2 to 5 tubes | 3. test group for 2 to 5 tubes |
fwdReverse: Pforward in reverse order and Preverse in normal order | 4. test group for 2 to 5 tubes | 5. test group for 2 to 5 tubes | 6. test group for 2 to 5 tubes |
revReverse: Pforward in normal order and Preverse in reverse order | 7. test group for 2 to 5 tubes | 8. test group for 2 to 5 tubes | 9. test group for 2 to 5 tubes |
bothReverse: Pforward and Preverse in reverse order | 10. test group for 2 to 5 tubes | 11. test group for 2 to 5 tubes | 12. test group for 2 to 5 tubes |