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Table 1 Pattern count on raw data (A and B), after a rough preprocessing step (C, D and E) to discard empty patterns or patterns containing obviously more than 2 cells on the first frame of the sequence. Eventually, the number of pattern where a transition from two to three cell was detected automatically (F) or manually (G). While a lower number of event is selected automatically, more than 80 % of the events selected automatically were also part of the manual selection whatever the experimental condition

From: Detection and tracking of overlapping cell nuclei for large scale mitosis analyses

 

Cyclophilin

LGN

p62

 

siRNA

siRNA

siRNA

A) Total number of fields of view (=large videos)

4

5

6

B) Total number of micropatterns (=single cluster videos)

1116

1393

1668

C) Micropatterns with no cells (excluded)

400

607

719

D) Micropatterns with too many cells at time 0 (excluded)

51

64

120

E) Micropatterns with a low number of cells at time 0

665

722

829

F) Events selected automatically from (E)

122

135

97

G) Events selected manually

184

197

227

H) Proportion of (F) also in (G)

85.9 %

82.5 %

81.8 %