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Fig. 1 | BMC Bioinformatics

Fig. 1

From: UNDR ROVER - a fast and accurate variant caller for targeted DNA sequencing

Fig. 1

Hi-Plex library structure and overlapping reads. The center rectangle represents the target insert DNA sequence flanked by gene-specific primer (GSP) sites (blue) and adapter sequences (green). The two reads of a pair are shown in yellow. The 5′ end of each read starts with its corresponding gene-specific primer sequence. The insert size is chosen so that both reads overlap the target insert sequence completely. The 3′ ends of reads may extend into the adapter sequence depending on the read length and the presence/absence of insertions/deletions in the template DNA. The diagram is not to scale. Typically, the insert sequence will be significantly longer than the primer sequences

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