Fig. 4

Comparison of expression estimates. a Expression estimates from Life Technologies (LifeTech) method and the default qpcR method are plotted against one another. The miRNA/sample combination in which the expression estimates differ most (miR-155; KW10:2) is represented by a black dot. An miRNA/sample combination in which the expression estimates roughly agree (miR-29a/KW10:2) is represented by a red dot. b & c The amplification curve data (ΔRn vs cycle) for b miR-155 or c miR-29a in sample KW10:2 with expression estimates for each method denote by vertical lines. Note that the amplification curve is still increasing at cycle 46 for miR-155 whereas for miR-29a is has leveled off