Fig. 2
From: Trimming of sequence reads alters RNA-Seq gene expression estimates
Influence of quality-based trimming on mappability. a The total number of input reads (light bars) and reads aligned to the transcriptome (dark bars) from four RNA-Seq data sets trimmed at a range of quality scores with SolexaQA. b The mappability, or number of aligned reads per total input reads per sample. Input reads shorter than 12 bases were not included in the calculation, as these are discarded by TopHat2 prior to alignment. Error bars represent standard deviations