Fig. 1
From: Trimming of sequence reads alters RNA-Seq gene expression estimates
High quality RNA-Seq data generated from D. melanogaster sensory neurons. a Confocal image of Drosophila larval sensory neurons expressing a nuclear-targeted version of mRFP (magenta) and a membrane-targeted version of GFP (white). Genotype: w 118 ; Gal4 21–7, UAS-mCD8-GFP/UAS-Red-Stinger. Scale bar is 100 μm. b Representative flow plots of D. melanogaster neurons. Plots show three progressive gates to identify RFP+ neurons, followed by two additional re-sorts with the same gates to assess purity. Compensated fluorescent values are shown. c Box plots generated in FastQC show average (blue lines) and median (red lines) quality scores across all read positions for each of the four independent replicate samples