Figure 4

Relationship between nuclei density and relative distance with respect to both nuclei-based differentiation measures. (A) Inverse nuclei density approach. Each degree of differentiation is given by an interval of nuclei density of the same width. Intervals of relative distance are compared. Due to the nearly exponentially decrease of the nuclei gradient, an interval of high nuclei density results in a small interval of relative distance. By contrast, an interval of small nuclei density leads to a large interval of relative distance. The equidistant method is illustrated in (B). The degree of differentiation is in the first place defined by the relative distance to the connective tissue. To equidistant intervals, the corresponding intervals of nuclei density are determined. Intervals of small distances lead to large intervals of nuclei density and intervals at larger distances result in narrow intervals of the nuclei density.