Figure 3
From: QuantPrime – a flexible tool for reliable high-throughput primer design for quantitative PCR
Primer pair details in QuantPrime. The figure shows an example of the 'Primer pair information' page. The page provides details about the selected primers and the amplicon. Positions to which the primers anneal within the target sequence are indicated in blue or green; the amplicon is highlighted by gray shadowing. Primers shown in blue anneal to an exon, whereas primers shown in green anneal across an exon-exon junction (the position of the intron is indicated by a red arrow head). In the 'Specificity test results' section, details about the specificity of the primer pair can be seen. If specificity problems exist, more details can be found here concerning the other possible amplicons.