Figure 2
From: QuantPrime – a flexible tool for reliable high-throughput primer design for quantitative PCR
'Results' in QuantPrime. The figure shows an example of the 'Results' page. Primer pairs successfully identified for the examined transcripts are presented. The following information is provided: the sequences (5' to 3') of the forward and reverse primers; the amplicon size (in bp); whether at least one primer spans an exon-exon junction ('Yes' in all cases in the example shown); the rank score (as calculated by Primer3); and the color code of the specificity rank given to the primer pair (see text for details). When clicking the primer pairs, more details are shown (see Figure 3).