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Table 2 Summary of errors and richness estimates when different criteria were used to merge OPFs. OPFs were merged when at least one peptide fragment in each OPF overlapped at least 5 aa and had a BSR value that was above the user specified level by the merge penalty. The type I error rate is the fraction of OPFs from the closed genome that correspond to multiple OPFs from the individual sequence reads. The type II error rate is the fraction of OPFs from the individual sequence reads that corresponded to more than one OPF from the closed genome sequence.

From: A statistical toolbox for metagenomics: assessing functional diversity in microbial communities

Merge Penalty

Type I Error Rate

Type II Error Rate

Observed Richness

Richness Estimation (True Richness = 3,730)

    

Chao1

ACE

Jackknife

Penalty = 0.00

0.063

0.129

2,927

3,038

2,976

3,137

Penalty = 0.05

0.067

0.100

3,223

3,332

3,271

3,413

Penalty = 0.10

0.071

0.074

3,462

3,574

3,510

3,653

Penalty = 0.15

0.080

0.056

3,642

3,757

3,691

3,839

Penalty = 0.20

0.091

0.046

3,810

3,925

3,858

4,004

No merge

0.719

0.003

11,538

11,668

11,616

11,968