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Figure 5 | BMC Bioinformatics

Figure 5

From: SpliceCenter: A suite of web-based bioinformatic applications for evaluating the impact of alternative splicing on RT-PCR, RNAi, microarray, and peptide-based studies

Figure 5

Output of batch siRNA-Check used for high-throughput analysis of siRNA or shRNA libraries. (a) The siRNA Position Report indicates the target location of each siRNA sequence, including gene symbol, chromosomal coordinates, and a link to a graphical view of the target gene. (b) The Hit/Miss report indicates which transcripts will, and which will not, be targeted by each siRNA sequence. (c) Summary statistics from a batch siRNA-Check analysis of the unique shRNA sequences of a 50,000+ shRNA library [23]. The chart indicates the percentage of shRNAs that do not target any currently annotated transcript, that target some but not all transcripts of the target gene, and that target all known splice variants of the target gene.

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BMC Bioinformatics

ISSN: 1471-2105

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