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Table 1 Estimated flux ratios from NMR measurements of S. cerevisiae.

From: An analytic and systematic framework for estimating metabolic flux ratios from 13C tracer experiments

flux ratios

our framework

METAFoR

PYR(mit) from MAL(mit) : PYR(mit) from PYR(cyt)

0.05 : 0.95

0.03 : 0.97

OAA(mit) from TCA-cycle : OAA(mit) from OAA(cyt)

0.50 : 0.50

0.50 : 0.50

PEP from OAA(cyt) : PEP from GA3P

0 : 1

0.04 : 0.96

OAA(cyt) from PYR(cyt) : OAA(cyt) from OAA(mit)

0.40 : 0.60

0.43 : 0.57

GLY from SER : GLY from C1 + CO2

0.96 : 0.04

0.96 : 0.04

  1. Directly comparable flux ratios computed from the NMR data by the framework presented in this paper and by manual flux ratio analysis (METAFoR).