Table 3 Comparison of functionally important motifs/residues conserved in GPR17 and related receptors
GPR17 | GPCR (type/family) | SHARED FEATURES (1) |
|---|---|---|
(residue number and structural domain) | Â | Â |
N49-D77-G290 TM1-TM2-TM7 | b Rh | 1.50–2.50–7.46 |
Y38-S287 TM1-TM7 | P2Y | 1.39–7.43 |
N114-D293 TM3-TM7 | P2Y | 3.35–7.49 |
H72-TRP156 TM2-TM4 | b Rh | 2.45–4.50 |
D128-R129 conserved interaction TM3-TM6 not conserved (3.50–6.30) | b Rh R of the DRY motif interacts with acidic residue in 6.30 (maintains the ground state) GPR17, P2Y, CysLT receptors have a basic residue in 6.30 | D/E-R-Y/W motif 3.49–3.50–3.51 |
A233-M236 | b Rh | Hydrophobic pocket accommodating DRY motif 2.33–2.36 |
H252-X-X-R255 TM6 | P2Y, CysLT receptors in P2Y agonists mediate receptor activation/coordination of the phosphate moiety | H-X-X-R motif 6.52-X-X-6.55 |
N77-N289-D293 D2.50-N7.45-D7.49 TM2-TM7 | b Rh | D2.50-N7.45-D7.49 TM7 residues belong to N/D-P-X-X-Y motif |
G10-L17 V173-L182 | b Rh | Plug β-hairpin in Nt and EL2 |
S118-water | b Rh | 3.39-water |
C104-C181 | All GPCR | disulphide bridge TM3-EL2 |
C23-C269 | P2Y, CysLT receptors | disulphide bridge Nt-EL3 |