Figure 2

MLPA normalization procedure. Panel A shows the peak intensities depending on probe sizes. Panel B shows the regression estimates for different methods: linear regression model using control probes (solid blue line), quadratic model using control probes (dotted blue line), nonlinear mixed model (red lines) using control probes (solid line) or median filter approach (dotted line). Panel C illustrates the parameters involved in the nonlinear mixed model. Panel D shows the size-adjusted normalized peak intensities prepared to compute the dosage quotient. In all panels dark lines represents control probes while light lines are for analytical probes.