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Table 1 Anova Table

From: FCI: an R-based algorithm for evaluating uncertainty of absolute real-time PCR quantification

Source

df

SS

MS

F.value

Regression

dfR = 1

SS R = ∑ i = 1 I J i ( y ^ i − y ¯ ¯ ) 2 MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaee4uamLaee4uam1aaSbaaSqaaiabbkfasbqabaGccqGH9aqpdaaeWbqaaiabbQeaknaaBaaaleaacqqGPbqAaeqaaOWaaeWaaeaacuqG5bqEgaqcamaaBaaaleaacqqGPbqAaeqaaOGaeyOeI0IafeyEaKNbaeHbaebaaiaawIcacaGLPaaadaahaaWcbeqaaiabikdaYaaaaeaacqqGPbqAcqGH9aqpcqqGXaqmaeaacqqGjbqsa0GaeyyeIuoaaaa@422B@

MS R = SS R df R MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaeeyta0Kaee4uam1aaSbaaSqaaiabbkfasbqabaGccqGH9aqpjuaGdaWcaaqaaiabbofatjabbofatnaaBaaabaGaeeOuaifabeaaaeaacqqGKbazcqqGMbGzdaWgaaqaaiabbkfasbqabaaaaaaa@38BA@

MS R s p 2 MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaqcfa4aaSaaaeaacqqGnbqtcqqGtbWudaWgaaqaaiabbkfasbqabaaabaGaee4Cam3aa0baaeaacqqGWbaCaeaacqqGYaGmaaaaaaaa@33EC@

Error

df E = ( I − 2 ) + ∑ i = 1 I ( J i − 1 ) MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaeeizaqMaeeOzay2aaSbaaSqaaiabbweafbqabaGccqGH9aqpdaqadaqaaiabbMeajjabgkHiTiabbkdaYaGaayjkaiaawMcaaiabgUcaRmaaqahabaWaaeWaaeaacqqGkbGsdaWgaaWcbaGaeeyAaKgabeaakiabgkHiTiabbgdaXaGaayjkaiaawMcaaaWcbaGaeeyAaKMaeyypa0JaeeymaedabaGaeeysaKeaniabggHiLdaaaa@42CA@

SS E = ∑ i = 1 I ∑ j = 1 J i ( y ij − y ^ i ) 2 MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaee4uamLaee4uam1aaSbaaSqaaiabbweafbqabaGccqGH9aqpdaaeWbqaamaaqahabaWaaeWaaeaacqqG5bqEdaWgaaWcbaGaeeyAaKMaeeOAaOgabeaakiabgkHiTiqbbMha5zaajaWaaSbaaSqaaiabbMgaPbqabaaakiaawIcacaGLPaaadaahaaWcbeqaaiabikdaYaaaaeaacqqGQbGAcqGH9aqpcqaIXaqmaeaacqqGkbGsdaWgaaadbaGaeeyAaKgabeaaa0GaeyyeIuoaaSqaaiabbMgaPjabg2da9iabbgdaXaqaaiabbMeajbqdcqGHris5aaaa@4A56@

MS E = SS E df E MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaeeyta0Kaee4uam1aaSbaaSqaaiabbweafbqabaGccqGH9aqpjuaGdaWcaaqaaiabbofatjabbofatnaaBaaabaGaeeyraueabeaaaeaacqqGKbazcqqGMbGzdaWgaaqaaiabbweafbqabaaaaaaa@386C@

 

   Lack of fit

dfL = (I – 2)

SS L = ∑ i = 1 I J i ( y ^ i − y ¯ i ) 2 MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaee4uamLaee4uam1aaSbaaSqaaiabbYeambqabaGccqGH9aqpdaaeWbqaaiabbQeaknaaBaaaleaacqqGPbqAaeqaaaqaaiabbMgaPjabg2da9iabbgdaXaqaaiabbMeajbqdcqGHris5aOWaaeWaaeaacuqG5bqEgaqcamaaBaaaleaacqqGPbqAaeqaaOGaeyOeI0IafeyEaKNbaebadaWgaaWcbaGaeeyAaKgabeaaaOGaayjkaiaawMcaamaaCaaaleqabaGaeeOmaidaaaaa@4390@

MS L = SS L df L MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaeeyta0Kaee4uam1aaSbaaSqaaiabbYeambqabaGccqGH9aqpjuaGdaWcaaqaaiabbofatjabbofatnaaBaaabaGaeeitaWeabeaaaeaacqqGKbazcqqGMbGzdaWgaaqaaiabbYeambqabaaaaaaa@3896@

MS L s p 2 MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaqcfa4aaSaaaeaacqqGnbqtcqqGtbWudaWgaaqaaiabbYeambqabaaabaGaee4Cam3aa0baaeaacqqGWbaCaeaacqqGYaGmaaaaaaaa@33E0@

   Pure error

df P = ∑ i = 1 I ( J i − 1 ) MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaeeizaqMaeeOzay2aaSbaaSqaaiabbcfaqbqabaGccqGH9aqpdaaeWbqaamaabmaabaGaeeOsaO0aaSbaaSqaaiabbMgaPbqabaGccqGHsislcqqGXaqmaiaawIcacaGLPaaaaSqaaiabbMgaPjabg2da9iabbgdaXaqaaiabbMeajbqdcqGHris5aaaa@3D84@

SS P = ∑ i = 1 I ∑ j = 1 J i ( y ij − y ¯ i ) 2 MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaee4uamLaee4uam1aaSbaaSqaaiabbcfaqbqabaGccqGH9aqpdaaeWbqaamaaqahabaWaaeWaaeaacqqG5bqEdaWgaaWcbaGaeeyAaKMaeeOAaOgabeaakiabgkHiTiqbbMha5zaaraWaaSbaaSqaaiabbMgaPbqabaaakiaawIcacaGLPaaadaahaaWcbeqaaiabbkdaYaaaaeaacqqGQbGAcqGH9aqpcqqGXaqmaeaacqqGkbGsdaWgaaadbaGaeeyAaKgabeaaa0GaeyyeIuoaaSqaaiabbMgaPjabg2da9iabbgdaXaqaaiabbMeajbqdcqGHris5aaaa@4A66@

s p 2 = SS p df p MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaee4Cam3aa0baaSqaaiabbchaWbqaaiabbkdaYaaakiabg2da9KqbaoaalaaabaGaee4uamLaee4uam1aaSbaaeaacqqGWbaCaeqaaaqaaiabbsgaKjabbAgaMnaaBaaabaGaeeiCaahabeaaaaaaaa@3979@

 

Total corrected

df T = ( ∑ i = 1 I J i ) − 1 MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaeeizaqMaeeOzay2aaSbaaSqaaiabbsfaubqabaGccqGH9aqpdaqadaqaamaaqahabaGaeeOsaO0aaSbaaSqaaiabbMgaPbqabaaabaGaeeyAaKMaeyypa0JaeeymaedabaGaeeysaKeaniabggHiLdaakiaawIcacaGLPaaacqGHsislcqaIXaqmaaa@3D88@

SS T = ∑ i = 1 I ∑ j = 1 J i ( y ij − y ¯ ¯ ) 2 MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGaee4uamLaee4uam1aaSbaaSqaaiabbsfaubqabaGccqGH9aqpdaaeWbqaamaaqahabaWaaeWaaeaacqqG5bqEdaWgaaWcbaGaeeyAaKMaeeOAaOgabeaakiabgkHiTiqbdMha5zaaryaaraaacaGLOaGaayzkaaWaaWbaaSqabeaacqqGYaGmaaaabaGaeeOAaOMaeyypa0JaeeymaedabaGaeeOsaO0aaSbaaWqaaiabbMgaPbqabaaaniabggHiLdaaleaacqqGPbqAcqGH9aqpcqqGXaqmaeaacqqGjbqsa0GaeyyeIuoaaaa@48F8@

  
  1. Analysis of variance for the linear regression model underlying the standard curve. In the absence of repeated measurements of ct for all standard dilutions the Error Sum of Squares (SSE) can not be broken up into Lack of fit (SSL) and Pure error (SSp) Sum of Squares. df, degrees of freedom; SS, Sum of Square; MS, Mean Square; y ^ i MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGafeyEaKNbaKaadaWgaaWcbaGaeeyAaKgabeaaaaa@2EE1@ , predicted value corresponding to xi; y ¯ i MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGafeyEaKNbaebadaWgaaWcbaGaeeyAaKgabeaaaaa@2EE9@ , mean of the replicated values of ct measured for the i-th standard; y ¯ ¯ = ∑ i = 1 I ∑ j = 1 J i ( y ij ) / ∑ i = 1 I J i MathType@MTEF@5@5@+=feaagaart1ev2aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacPC6xNi=xH8viVGI8Gi=hEeeu0xXdbba9frFj0xb9qqpG0dXdb9aspeI8k8fiI+fsY=rqGqVepae9pg0db9vqaiVgFr0xfr=xfr=xc9adbaqaaeGacaGaaiaabeqaaeqabiWaaaGcbaGafeyEaKNbaeHbaebacqGH9aqpdaWcgaqaamaaqahabaWaaabCaeaadaqadaqaaiabbMha5naaBaaaleaacqqGPbqAcqqGQbGAaeqaaaGccaGLOaGaayzkaaaaleaacqqGQbGAcqGH9aqpcqqGXaqmaeaacqqGkbGsdaWgaaadbaGaeeyAaKgabeaaa0GaeyyeIuoaaSqaaiabbMgaPjabg2da9iabbgdaXaqaaiabbMeajbqdcqGHris5aaGcbaWaaabCaeaacqqGkbGsdaWgaaWcbaGaeeyAaKgabeaaaeaacqqGPbqAcqGH9aqpcqqGXaqmaeaacqqGjbqsa0GaeyyeIuoaaaaaaa@4C95@