PASS predicted scores of well-studied poly(A) signals and the relative efficiencies of polyadenylation signal mutants of rbcS determined by wet experiments. The red triangles denote validated poly(A) sites in the wild type mRNA. A. PASS scan of CaMV 35S RNA 3'-UTR [14, 28], which is widely used as a polyadenylation signal for transgene expressions. B. Wild type 3'-UTR of rbcS profile scanned by PASS. The authenticated poly(A) sites are as marked. The predicted scores and the actual efficiencies of each site being used are tightly associated in which sites 2 and 3 are the major ones, while sites 1 and 4 are minor ones . C. A set of representative poly(A) signal mutations of the site 1 of rbcS and their predicted scores by PASS. The dash line indicates the poly(A) site positions. D. Relationship between predicted score and poly(A) site 1 efficiencies of the mutants shown in C. The poly(A) site efficiency data were extracted from the results presented by Li and Hunt (; Figures 2 to 4 therein).