Development of an epitope conservancy analysis tool to facilitate the design of epitope-based diagnostics and vaccines

Table 1 Example conservancy analysis of a continuous sequence

	Reference sequence¹										Identity²
Source	F	L	P	S	D	F	F	P	S	V	No.	(%)
Strain 1	Y	L	P	S	D	F	F	P	S	I	8	(80)
Strain 2	F	L	P	S	D	F	F	P	S	V	10	(100)
Strain 3	R	L	P	S	K	Q	F	P	S	V	7	(70)
Strain 4	Y	E	P	T	D	F	F	P	S	V	7	(70)
Strain 5	F	L	P	T	D	F	F	P	S	V	9	(90)
Strain 6	F	L	P	T	D	F	S	F	T	V	6	(60)
Strain 7	F	L	P	S	D	F	F	P	S	V	10	(100)
Strain 8	Y	E	P	S	E	F	S	F	S	I	4	(40)
Strain 9	F	L	P	S	E	F	F	P	S	V	9	(90)
Strain 10	F	L	P	S	D	F	F	P	S	V	10	(100)
Total: conservancy at identity threshold ≥ 80%											6	(60)
Total: variability at identity threshold <80%											4	(40)

1. Residues that are different from that of the corresponding residue in the reference sequence are highlighted in bold.
2. Identity indicates the number (%) of residues in the homologous sequence that are identical to the corresponding residue in the reference sequence.
3. Totals indicate the number (%) of strains in which the reference sequence is found with an identity above or below the indicated threshold.

ISSN: 1471-2105