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Table 3 Sensitivity of compound-feature combinations to cell segmentation

From: Impact of image segmentation on high-content screening data quality for SK-BR-3 cells

  

Resistant

Sensitive

Overall Sensitive %

Channel

Feature Type

   

Ch1

arrangement

24

16

58%

 

dimension

16

94

 
 

intensity

32

18

 
 

shape

33

27

 
 

texture

14

6

 

Ch2

intensity

19

1

5%

Ch3

arrangement

6

14

47%

 

intensity

36

14

 
 

texture

26

33

 

Ch4

arrangement

10

20

46%

 

dimension

7

3

 
 

intensity

57

33

 
 

shape

2

8

 
 

texture

32

28

 
  1. The dose response of each feature for each drug was fitted by a linear model, using either the well-segmented cell population, or the poorly-segmented cell population. The discrepancy between the well- and poorly- segmented dose responses was calculated for each drug-feature combination, and drug-feature combinations were then binned as either sensitive to segmentation (if the discrepancy was larger than the grand median discrepancy) or resistant to segmentation (if the discrepancy was smaller than the grand median).