Figure 5From: Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomicsEvaluation of the classification performances of SFOER and PeptideProphet with standard protein mixture. A) Number of correct and incorrect peptide identifications by SFOER and PeptideProphet under different FDR, where incorrect peptide identification indicates peptide assignment from forward yeast database while correct one is from known standard proteins and trypsin. B) Predicated and observed FDRs. Observed FDR is calculated as the number of peptide identifications not from standard proteins over total peptide identifications, while predicated FDR is calculated using equation (1). Observed FDR for SFOER are presented by open circles, while observed FDR for PeptideProphet are represented by filled circles.Back to article page