Figure 1

In this figure, interactions identified by analysis but not previously reported are observed to have potential biological interest. Genes and gene products are ovals, solid arrows are identified, directed interactions, dashed lines with arrows are more general interactions implied by this subnetwork. Purple box identifies those proteins that physically interact to form a functional unit. In this figure, cell cycle-regulated transcription factors (yellow) and protein kinase KCC4 concurrently regulates HSL1 and MNN1. MNN1 subsequently regulates PMT1-5. KCC4 and HSL1 (orange) are genes that associate with morphogenesis, septin checkpoints, and bud neck [32, 38-40]. MNN1 and PMT1-5 [green] are mannosyltransferases [41, 42]. These seemingly unrelated groups of genes and functions are united by the observation of Gladfelter, et al. [43] that the cell wall at the base of the bud is derived from mannose-rich glycoproteins that are delivered through the secretory pathway, and they suggest that septins target secretory vesicles to the base of the bud. Thus, the sub network identified here uniting cell cycle-regulating transcription factors with bud neck and septin checkpoint and mannosyltransferases, though not explicitly previously identified, is consistent with previous biological observations.