Figure 2

Sample Relos outputs. Relos was used to generate and analyze promoters using four different models. Five thousand promoters were generated in all Figure 2 simulations. A. A simulation that depicts a single activator, modeled as an additive rule. B. A simulation that depicts an activator and a repressor modeled as additive rules. C. A simulation that depicts a synergistic rule between two regulatory elements. Each element has a small additive contribution to expression, but promoters with at least one of each element have enhanced expression. Gaussian noise was added to the output of the simulation at 5% of the level of expression of individual promoters. D. A simulation that depicts a cooperative interaction between two regulatory elements modeled with a hill function. Noise was added to the simulation as in C.