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Table 4 Error analysis. Four sources of bias were considered: average identity between equivalent isoforms, AS mechanism, size of the insertion/deletion, and sequence change location, which can be external (AS involves at least one sequence terminus), internal (no sequence terminus is affected by AS) and external+internal (AS changes happen at both external and internal locations). Whilst a certain trend may be observed for the first case, the performance of the method is nonetheless high. Only small performance changes are observed for AS mechanism, size of the insertion/deletion andsequence change location.

From: A procedure for identifying homologous alternative splicing events

Average identity between equivalent isoforms (sample size)

Accuracy

Precision

Sensitivity

90% (N = 305)

0.99 ± 0.00

0.96 ± 0.02

0.95 ± 0.01

80% (N = 89)

0.98 ± 0.02

0.89 ± 0.06

0.89 ± 0.06

70% (N = 25)

0.93 ± 0.04

0.83 ± 0.05

0.83 ± 0.05

60% (N = 13)

0.95 ± 0.06

0.83 ± 0.24

0.83 ± 0.24

AS mechanism (sample size)

Accuracy

Precision

Sensitivity

Insertions/deletions (N = 248)

0.98 ± 0.01

0.91 ± 0.03

0.90 ± 0.02

Substitutions (N = 147)

0.99 ± 0.01

0.96 ± 0.04

0.95 ± 0.03

Complexes (N = 78)

1.00 ± 0.00

0.99 ± 0.02

0.99 ± 0.02

Insertion/deletion size (sample size)

Accuracy

Precision

Sensitivity

Small (N = 145)

0.98 ± 0.00

0.90 ± 0.01

0.90 ± 0.00

Big (N = 103)

0.98 ± 0.01

0.91 ± 0.05

0.91 ± 0.05

AS region position (sample size)

Accuracy

Precision

Sensitivity

External (N = 153)

0.99 ± 0.01

0.95 ± 0.06

0.95 ± 0.06

Internal (N = 235)

0.99 ± 0.01

0.91 ± 0.02

0.90 ± 0.00

External+Internal (N = 85)

1.00 ± 0.01

0.99 ± 0.02

0.99 ± 0.02